熒光光纖氧氣測量技術(shù)具有高精確度、高可靠性、響應(yīng)時間短、適用于氣相和液相等優(yōu)勢，因此隨著技術(shù)的問世，精確、高通量測量微小生物的呼吸和評估其能量代謝成為可能。高通量呼吸測量系統(tǒng)基于熒光光纖氧氣測量技術(shù)，能夠?qū)壍任⑿⌒屠ハx、蟲卵、蛹、線蟲、土壤動物等微小型無脊椎動物進(jìn)行測量，測定其耗氧量，進(jìn)而評估其代謝水平。系統(tǒng)在昆蟲生理生態(tài)學(xué)、比較生物學(xué)、實(shí)驗(yàn)生物學(xué)、污染生態(tài)學(xué)與環(huán)境毒理學(xué)、環(huán)境科學(xué)、氣候變化研究等領(lǐng)域具有越來越重要的應(yīng)用價值。
果蠅卵、幼蟲、蛹、成蟲的耗氧率測定
左圖：果蠅卵、幼蟲、蛹耗氧率的比較；右圖：果蠅成蟲耗氧率（麻醉處理VS對照）
系統(tǒng)由內(nèi)置熒光光纖氧氣傳感器的微型呼吸室、氧氣測量主機(jī)及數(shù)據(jù)采集分析軟件組成，可對96個通道的樣品進(jìn)行同步測量。
功能特點(diǎn)
- 氧氣測量高精度、高可靠性、低功耗、低交叉敏感性、快速響應(yīng)時間
- 輕松校準(zhǔn)
- 非侵入性和非破壞性測量
- 緊湊設(shè)計，適用于溫控培養(yǎng)箱和/或搖床
- 氣體氧和溶解氧均可測量
技術(shù)參數(shù)
- 檢測技術(shù)：光纖氧傳感器技術(shù)。
- 適用場景：原位檢測，可在培養(yǎng)箱里或搖床上使用，便于溫度控制。
- 呼吸室：透明聚苯乙烯材質(zhì)，支持預(yù)消毒處理，可重復(fù)使用。
- 氧氣測量主機(jī)：單個重670 g，162 x 102 x 32 mm
- 主機(jī)內(nèi)置溫度傳感器：0-50°C，分辨率012°C，精度±0.5°C
- 主機(jī)內(nèi)置壓強(qiáng)傳感器：300-1100mbar，分辨率11mbar，精度±6mbar
- 最大采樣頻率：單通道激活時可達(dá)10-20次每秒
- 氧氣測量精度：±0.1% O2@1% O2或±0.05 mg/L@0.44 mg/L
- 氧氣測量分辨率：01% O2@1% O2或0.005 mg/L@0.44 mg/L
- 電源：5VDC，USB供電
- 響應(yīng)時間＜30s
- 通道數(shù)：96
左圖：封閉呼吸室中的苜蓿切葉蜂子脾和蛹；右圖：高通量呼吸系統(tǒng)和傳統(tǒng)呼吸測量法的結(jié)果比較
苜蓿切葉蜂耗氧率（V?O2）隨溫度的變化曲線
參考文獻(xiàn)
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